Powered by biomethane gas, the Bio-Bus will use waste from more than 32,000 North Berwick and Haddington households along its 5-mile route.
Operated by Hunter Gowk, the bus will fill up at a site in Longniddry, where sewage and inedible food waste is turned into biomethane gas.
The bus, which can seat up to 40 people, is to be unveiled on 1st April in Dunbar.
If the scheme is successful, Hunter Gowk will consider introducing more “poo buses”. The managing director, Avril Fish, said: “From being a design concept, on our drawing board in our Musselburgh office last year, the Bio-Bus has become a fully-fledged product and we are very excited about bringing it to East Lothian”.
Timetables for the Bio Bus can be seen here.